Electrophoresis is a method used to separate molecules based on their charge and size. Used in studies such as purification, molecular weight determination, or characterization, this method involves loading the sample to be tested into a porous matrix and, using an applied voltage, moving the molecule through the matrix according to its properties.
The homogeneity of proteins in pharmaceutical formulations can be determined and evaluated using analytical gel electrophoresis. With this technique, it is possible to calculate the molecular weights of protein subunits and determine the subunit structure of pure proteins.
Electrophoresis is a frequently used method for the qualitative characterization of large molecule active pharmaceutical ingredients, the detection of impurities for health control, the performance of quantitative determinations and the separation of related impurities.
In our laboratory, electrophoresis studies and method validation can be carried out based on existing pharmacopoeia methods, monographs and literature research for the product and active pharmaceutical compound to be tested, or based on methods provided by the customer.